Maceration Mediated Extraction of Marijuana Leaves Oil and Separation of Δ9-Tetrahydrocannabivarin (THCV) by Thin Layer Chromatography

Shazia Zulfqar; Gul Bahar  Ali; Soha  Jamal; Shafqat  Munir; Alia  Muqqddus

doi:10.55927/ajns.v2i4.4223

Authors

Shazia Zulfqar Minhj University Lahore
Gul Bahar Ali International Islamic University Islamabad
Soha Jamal Minhj University Lahore
Shafqat Munir Minhj University Lahore
Alia Muqqddus Government College University Faslabd

DOI:

https://doi.org/10.55927/ajns.v2i4.4223

Keywords:

Extraction, Marijuana Leaves, Maceration Mediation, (THCV), Chromatography

Abstract

Cannabidiol (CBD), non-psychoactive cannabinoid, was extracted from Cannabis sativa leaves by using solvent extraction method followed by reflux condenser procedure. Physical and chemical properties of Cannabidiol (CBD) were determined. Various parameters and contents of extracted oil were analyzed. Two different samples were prepared by applying different conditions. Sample “A” was prepared by applying reflux condenser and sample “B” was prepared by soaking eaves in ethanol (solvent) for one week. Various parameters and contents of extracted oil were analyzed. TLC, UV, GC-MS, IR and HPLC techniques were applied for characterization of both samples and oil. Some tests were also performed for the detection of proteins, carbohydrates, Steroids, tannins, gums and mucilage. Acid value, saponification number and specific gravity were also calculation by applying titration method

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