Activity Test of the Preparation of Dental Passage Gel Extract of Basil Leaf Ethanol (Ocimum Basilicum L.)  Againts Staphylococcus Aureus

Rosita Irianti Dehi; Alvernia Dwi Maulana

doi:10.55927/ijis.v1i4.3490

Authors

Rosita Irianti Dehi Department of Pharmacy, Health Polytechnic, Ministry of Health, Jayapura
Alvernia Dwi Maulana Department of Pharmacy, Health Polytechnic, Ministry of Health, Jayapura

DOI:

https://doi.org/10.55927/ijis.v1i4.3490

Keywords:

Basil Leaf (Ocimum Basilicum L.) Extract, Dental Passage Gel, Staphylococcus Aureus

Abstract

Based on this, basil leaves are formulated in gel toothpaste preparations that have antibacterial activity.This study aims to determine the antibacterial activity of basil leaf gel toothpaste with various baseh concentrations, 20%, 40% and 60% and to determine the best concentration of basil leaf extract in gel toothpaste preparations that have antibacterial activity against Staphylococcus aureus. Basil leaves were extracted by maceration method using 96% ethanol solvent which was then tested for antibacterial activity using disc diffusion method with Ciprofloxacin as a positive control and aquadest as a negative control. The results of antibacterial testing showed that the mouthwash with base concentration did not produce an inhibition zone, 20% had a diameter of 6.68 mm, a concentration of 40% had a diameter of 7.81 mm and 60% had a diameter of 8.77 mm. The greater the concentration, the larger the resulting inhibition zone. In this study, it can be concluded that the gel toothpaste of basil leaf extract (Ocimum basilicum L.) which can inhibit the Staphylococcus aureus is at a concentration of 60% with a moderate inhibitory response category

Downloads

Download data is not yet available.

References

Adelberg, Jawetz, & Melnick. (2008). Medical Microbiology. Jakarta: EGC Medical Book Publishers.

Agatha Maylie Wijatno. (2014). Use of Carbomer As a Gelling Agent in a Toothpaste Formula of Guava Leaf Ethanol Extract (Psidium Guajaval.) .

Alamsyah HK, Widowati I, S. A. (2014). Effectiveness of White Fruit Flesh Guava Leaf Extract (Psidium guajava Linn) at Concentrations of 5%, 10%, and 15% against zone 39 http://repository.unimus.ac.id radical bacteria Staphlococcus aureus. Antibacterial Activity of Sargassum Cinereum (J.G.Agardh) Seaweed Extract From Jepara Long Island Waters Against Escherichia Coli Bacteria and Staphylococcus Epidermidis, Journal of Marine Research, 3, pp. , 69–78. https://eprints.umm.ac.id/47530/3/BAB II.pdf

Allen, L., V. (2002). Liquid and semisolid Pharmaceutical Preparations. ITB Publishers, Bandung, 127. http://ejurnal.undana.ac.id/index.php/CMJ/article/view/4940.

Angelika, G. P., Agung, S., & Pujiyanto, S. (2014). Antibacterial Activity Test of Euphorbia hirta L. Plant Extract Against Ralstonia solanacearum, Escherichia coli, And Staphylococcus aureus In Vitro. Journal of Biology, vol 3(2), 49–58.

Ariani, N., & Ayuchecaria, N. (2019). "Evaluation of the Level of Adherence to Taking Hypertension Medications in Patients of the Refer-Back Program at Banjarmasin Partner Pharmacies". Ibn Sina Scientific Journal, 4(2), 452–459. Retrieved from http://e-jurnal.stikes- isfi.ac.id/index.php/JIIS/article/view/359.

Aslamnur Fikri Ramadhana., Rini Hendriani. 2020. Problems And Development Of Drug Formulations For Children's Dosing Forms.

Azadeh M, et al. (2011). The Profile Of Pathogenic Isolated From Dental Plaque-Indunceed Gingivitis. International Journal of Molecular and Clinical Microbiology, p, 36–39.

Badruttamam, M. I., A. H. Baihaqi, D. F. Islamiyanti, Koimah, N. R. Ati, I. S. (2016). Identification of Bacteria in the Mouth. Available on https://www.scribd.com/document/332711198/Identifikasi-Bakteri-Pada- Mouth. Retrieved December 28, 2019. jurnal.untan.ac.id

Hadipoentyanti, E. & Wahyuni , S. (2008). Diversity of basil (Ociumum Spp.) based on morphological character, production and quality of herbs. Journal of Littri, 14(4), 141–148. jurnal.untan.ac.id

Handayani, H., and F. H. S. (2016). Antioxidant Extraction of Soursop Leaves Ultrasonic Bath Method.

Helni Maulida. (2018). TEST OF ANTIBACTERIAL EFFECT OF BASIL LEAF ETHANOL EXTRACT (Ocimum tenuiflorum L.) AGAINST THE GROWTH OF Staphylococcus aureus bacteria.

Kurniawan B and Aryana W F. (2015). Effectiveness of White Fruit Flesh Guava Leaf Extract (Psidium guajava Linn) at Concentrations of 5%, 10%, and 15% against zone 39 http://repository.unimus.ac.id radical bacteria Staphlococcus aureus. Binahong (Cassia alata L) as inhibitor of Escherichia Coli Growth, Majority, 4(4), pp. , 100–104. https://eprints.umm.ac.id/47530/3/

Melinda, 2014. (2014). Antibacterial Activity of Henna Leaves (Lowsonia Inermis L.). Thesis, Muhammadyah Surakarta University, Surakarta.

Mitsui, T. (1997). New Cosmetic Science. Tokyo: Elsevier, p 486.

Najlah, F. (2010). Effectiveness of White Fruit Flesh Guava Leaf Extract (Psidium guajava Linn) at Concentrations of 5%, 10%, and 15% against zone 39 http://repository.unimus.ac.id radical bacteria Staphlococcus aureus. Thesis. Faculty of Medicine and Health Sciences, Muhammadiyah University Yogyakarta. https://eprints.umm.ac.id/47530/3/BAB II.pdf

Natasya Detami Sheline Ballo, Desi Indriarini, A. L. S. A. A. (2021). Liquid and semisolid Pharmaceutical Preparations. ITB Publishers, Bandung, 127. http://ejurnal.undana.ac.id/index.php/CMJ/article/view/4940.

Quelab. (2005). Mc Farlands Standards. Avalaible at www.quelab.com/. Retrieved 14 April 2011.

Roby Dalinatola. (2016). The Inhibitory Power of Betel Nut Seed Extract (Areca catechu L.) Against the Growth of Staphylococcus aureus Bacteria. 14–16.

Rundengan, C. H. (2017). Inhibition Test of Yaki Betel Nut Ethanol Extract (Areca vestiaria) against Stapyhlococcus aureus Bacteria, Escherichia coli, Pseudomonas aeruginosa. Pharmacon, 6(1).

Rosida, Sidiq, H.B.H.F. and Apriliyanti, I. P. (2014). Evaluation Of Physical Properties And Irritant Test Of Banana Peel Extract Gel (Musa acuminata Colla). Journal of Current Pharmaceutical Sciences,. 2018, Volume 2(1, 131–135.

Rowe, R. C. et al. (2009). Handbook Of Pharmaceutical Excipient, 6th Ed. The Pharmateutical Press, London.

Storehagen, S., & Midha, N. O. S. (2003). Dentifrices And Mouthwashes Ingredients And Their Use. Seksjon For Odontologik Pharmacology Og Pharmacotherapy, Institute For Klinisk Odontology, Det Odontologiske Fakultet. Universitetet I Oslo.

Sudarsono, D. (2002). In Medicinal Plants II. Gadjah Mada Sekip University, p. 41.