Antibacterial and Antibiofilm Activity Related to the Mechanism of Work of the Active Fraction of Moringa Leaves (Guilandina Moringa L.) to  Staphylococcus Aureus

Armayanti Rindiarko Hasbrianti; Opstaria; Wiwin Herdwiani

doi:10.55927/mudima.v4i8.11134

Authors

Armayanti Rindiarko Hasbrianti Department of Pharmaceutical Science, Faculty of Pharmacy, Setia Budi University, Surakarta
Opstaria Department of Pharmaceutical Science, Faculty of Pharmacy, Setia Budi University, Surakarta
Wiwin Herdwiani Department of Pharmaceutical Science, Faculty of Pharmacy, Setia Budi University, Surakarta

DOI:

https://doi.org/10.55927/mudima.v4i8.11134

Keywords:

Antibiofilm, Roselle Flowers, E.Coli, Hibiscus Sabdariffa L

Abstract

Biofilm-associated S. aureus bacterial infections are difficult to treat because bacterial cells encased in biofilms can be highly resistant to antibiotics and the immune response of the host. Moringa leaf extract contains active compounds of tannins, saponins, alkaloids, and phenols that have activity as antibacterial and antibiofilm. This study aims to determine the antibacterial activity and of extracts, mechanism of action of the most active fraction of moringa leaf ethanol extract against S. aureus.Extraction of Moringa leaves using maceration method with 96% ethanol solvent, fractionated using n-hexane, ethyl acetate, and water solvents. Antibacterial test using disc diffusion method, followed by dilution test. Observations were made using SEM and AAS. Data from the diffusion test, inhibition of biofilm formation, and biofilm degradation were analyzed using One Way ANOVA. Based on the results of the study, the ethyl acetate fraction is the most active fraction in inhibiting the growth of S. aureus in the diffusion test with an inhibition zone diameter of 15,7 mm at a concentration of 100 mg/mL and a minimum kill concentration value of 25 mg/mL. The water fraction was the most active fraction in inhibiting the formation and degradation of biofilm against S. aureus with an IC50 value of 4,049 ± 0,063 mg/mL and an EC50 value of 4,246 ± 0,050 mg/mL

References

A.Amalia Dahlia, Asni Amin, R.L., & Fakultas. (2012). Identification of Morphology and Specific Parameters of Simplicia and Rosella (Hibiscus Sabdariffa L.) Leaf Extract from Kab. Enrekang (South Sulawesi). 04(02), 159–175.

Alhede, M., Alhede, M., Qvortrup, K., Kragh, K. N., Jensen, P. Ø., Stewart, P. S., & Bjarnsholt, T. (2020). The Origin Of Extracellular Dna In Bacterial Biofilm Infections In Vivo. Pathogens And Disease, 78(2), 1–15.

Ali, K., Yuziani, & Mulyati Sri Rahayu. (2022). Activity Test of Ethanol Extract of Breadfruit Leaves (Artocarpus Altilis) Against Escherichia Coli Bacteria. Human and Health Scientific Journal, 5(2), 265–271.

Alvita, L. R., Falah, S., & Nurhidayat, N. (2017). Water Extract Activity Of Papaya Leaf As Antibiofilm Against Escherichia Coli. Current Biochemistry, 2(3), 164–175. Https://Doi.Org/10.29244/Cb.2.3.164-175

Andayani, N., Nurhayati, D., & Saing, M. D. (2022). Optimization of Bacterial Growth E. Coli and Bacillus Subtilis in Edamame Media to Optimize the Growth of E. Coli And Bacillus Subtilis. 1(1), 45–53.

Arjuna, A., Pratama, W. S., Sartini, S., & Mufidah, M. (2018). Anti-Biofilm Preliminary Test of Green Tea and Black Tea Extracts in Streptococcus Mutans Via the Microtiter Plate Method. Galenika Journal Of Pharmacy (E-Journal), 4(1), 44–49.

Basavaraju, M. (2022). Escherichia Coli: An Overview Of Main Characteristics. November.

Brooks, G. F., Butel, J. S., & Ornston, L. N. (2008). Jawetz, Melnick And Adelberg Medical Microbiology (Translation). EGC Medical Books, Jakarta, 627–629.

Chofidah, A. I., Danu, M. D., & Rosyidah, I. H. (2019). Test Antibacterial Activity of Roselle Kombucha (Hibiscus Sabdariffa L.) Against the bacteria Escherichia Coli and Staphylococcus Aureus, S1 Pharmacy Study Program, Anwar Medika Hospital Stickers, Sidoarjo, East Java, D3 Pharmacy Study Program, Hospital Stickers. 2(1), 43–47.

Estri, M. R., & Anggarbeni, S. R. (2015). Test of the Inhibitory Power of Water Boiled from Rosella Flowers (Hibiscus Sabdariffa L.) Against the Growth of Esherichia Coli Bacteria. Wiyata Journal, 2, 9–13.

Fathoni, M. M., Isnaeni, I., & Darmawati, A. (2021). Anti-Bacterial Activity Of Rosela Flower Extract (Hibiscus Sabdariffa L.) Against Extended-Spectrum Beta-Lactamase (Esbl) Eschericia Coli. Scientific Periodicals in Pharmaceutical Chemistry, 8(1), 7.

Fiana, F. M., Kiromah, N. Z. W., & Purwanti, E. (2020). Antibacterial Activity of Ethanol Extract of Breadfruit Leaves (Artocarpus Altilis) Against Bacteria Staphylococcus Aureus And Escherichia Coli. Pharmacon: Indonesian Pharmacy Journal, 10–20.

Fink, R., & Filip, S. (2023). Surface-Active Natural Saponins . Properties , Safety , And Efficacy. International Journal Of Environmental Health Research, 33(7), 639–648.

Fitriana, Y. A. N., Fatimah, V. A. N., & Fitri, A. S. (2020). Anti-Bacterial Activity of Betel Leaves: Extract Test for Khm (Minimum Inhibitory Level) and Kbm (Minimum Bactericidal Level). Saintex, 16(2), 101–108.

Hardyanti Eka Putri. (2016). Uji TAcute toxicity of Ethanol Extract of Bidara Leaves (Ziziphus Spina-Christi L.) TThe Morphology and Histology of Mice Liver (Mus Musculus) Male. X, 1–21.

Hermawati, A. H., Surtini, S., Arohman, A. L., & Hariyanto, H. (2023). Ciprofloxacin Antibiotic Test on Growth Escherichia Coli In Vitro. Scholarly Journal, 10(3), 181–188.

Isnaini, L. (2010). Extraction of Natural Liquid Red Colorant Containing Antioxidant From Rosella (Hibiscus Sabdariffa L) Flower Petals and Its Application in Food Products The Extraction of Natural Liquid Red Colorant Containing Antioxidant From Rosella (Hibiscus Sabdariffa L) Calyx And. Journal of Agricultural Technology, 11, 18–27.

Jaddoa, N. T. M., & Gharb, L. A. (2021). The Antibiofilm Activity Of Hibiscus Sabdriffa L. Against Methicillin-Resistant Staphylococcus Aureus. Iraqi Journal Of Agricultural Sciences, 52(3), 626–631.

Miquel, S., Lagrafeuille, R., Souweine, B., & Forestier, C. (2016). Anti-Biofilm Activity As A Health Issue. Frontiers In Microbiology, 7(Apr), 1–14.

Muslihin, A. M., & Budiyanto, A. B. (N.D.). Determination of water soluble essence content, ethanol soluble essence content and identification of alkaloids in 96% ethanol extract of cherry leaves (Muntingia Calabura L.).

Nahor, E. M., Rumagit, B. I., & Tou, H. Y. (N. D.). Comparison of the Yield of Ethanol Extract of Andong Leaves (Cordyline Futicosa L.) Using Maceration and Sochletation Extraction Methods 40–44.

Nopiyanti, V., & Aisiyah, S. (2020). Test for Determining the Spf Value (Sun Protection Factor) of Roselle Flower Fractions (Hibiscus Sabdariffa L.) As an Active Sunscreen Substance D. Journal Of Pharmacy, 9(1), 2656–8950.

Novita, W. (2016a). Against the Growth of Streptococcus Mutans Willia Novita Bacteria. jmj, 4.

Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Comparison of Yogurt Starter Antibacterial Activity Testing Using the Well Diffusion Method and the Disc Diffusion Method. Journal of Animal Products Technology, 1(2), 41.

P, D. P. S., Nur, Y., Fadraersada, J., Farmasi, F., & Mulawarman, U. (2018). Stability Profile of Rosella (Hibiscus Sabdariffa) Flower Extract as a Lipstick Colorant Candidate. November, 20–21.

Purba, H., Simanjuntak, H. A., & Situmorang, R. (2020). Phytochemical Screening Of Rosella Flowers (Hibiscus Sabdariffa L) And Antimicrobial Activity Test. Journal of Chemical Education, 12(2), 70–78.

Putri, F. E., Diharmi, A., & Karnila, R. (2023). Identification of Secondary Metabolite Compounds in Brown Seaweed (Sargassum Plagyophyllum) Using the Fractionation Method. Journal of Indonesian Agricultural Technology and Industry, 15(1), 40–46.

Ridwan, M., Risandiansyah, R., Yahya, A., Ridwan, M., Risandiansyah, R., & Yahya, A. (N.D.). Antibacterial and Antibiofilm Activity of Rosella (Hibiscus Sabdariffa) Petal Extract Against Streptococcus Mutans 1–11.

Rollando. (2017). Isolation, Identification, Characterization, and Testing of Gallic Acid Derivative Antibiofilm. 7(2), 105–111.

Samrot, A. V., Mohamed, A. A., Faradjeva, E., Jie, L. S., Sze, C. H., Arif, A., Sean, T. C., Michael, E. N., Mun, C. Y., Qi, N. X., Mok, P. L., & Kumar, S. S. (2021). Mechanisms And Impact Of Biofilms And Targeting Of Biofilms Using Bioactive Compounds—A Review. Medicine (Lithuania), 57(8), 1–28.

Soegianto, L., Hertiani, T., & Pramono, S. (2016). Isolation and Identification of Antibacterial and Antiquorum Sensing Substances in Roselle Flower Petal Extract (Hibiscus Sabdariffa L). Indonesian Journal of Pharmaceutical Sciences, 14(2), 212–218.

Sudarwati Dwi, & Sumarni Woro. (2016). Test of Antibacterial Compound Activity in Moringa Leaf and Rosella Flower Extracts. Indonesian Journal Of Chemical Science, 5(1), 1–4.

Sugito, S., & Marliyana, S. D. (2021). Performance Test of the Thermo Ice 3000 Atomic Absorption Spectrophotometer on Pb Metal Using Crm 500 and Crm 697 at the Uns Integrated Laboratory. Indonesian Journal Of Laboratory, 4(2), 67.

Suwadi, P., Fauzan, R. D., Yulianto, A., Usman, A. N., & Fauzi, A. (2021). Diversification of Rosella Plants (Hibiscus Sadbariffa L.) as an Effort to Improve the Welfare and Economy of the Community of Sumberdem Village, Wonosari, Malang. Semar (Journal of Science, Technology and Arts for Society), 10(1), 22.

Syarifuddin, A., Sulistyani, N., & Kintoko, K. (2018). Activity Of Antibiotic Bacterial Isolate Kp13 And Cell Leakage Analysis Of Escherichia Coli Bacteria. Indonesian Journal of Pharmaceutical Sciences, 16(2), 137.

Widarta, I. W. R. (2020). Patron Prof. Dr. Ir. I Ketut Satriawan, Mt Responsible Prof. Ir. I Nyoman Semadi Antara, Ph. D Chief Editor Gede Arda, Stp., M. Sc. Internal Reviewer Dr. Ir. Dewa Gde Mayun Permana, Mp. Dr. I Wayan Arnata, S. But ., M . The External Reviewer Managing Editor N . L . Ari Yusasrini, Stp., Mp. Production and Distribution I Kadek Adiguna, Se Ni Kadek Pindari, S. Comm. 5(2).

Widnyani, I. A. P. A., Yoga, W. K. Y., Ariani, N. K. S., & Sintyadewi, P. R. (2023). Processing of Rosella (Hibiscus Sabdariffa) Flowers as a Syrup Product with Antioxidant Content at SMA Negeri 1 Rooftop Lembongan. Widya Laksana Journal, 12(2), 204–211.