Isolation and Characterization of a Silicate-Solubilizing Bacterial Strain Associated with the Roots of Groundnut (Arachis hypogaea L.)
DOI:
https://doi.org/10.55927/ijaea.v2i1.3589Keywords:
Rhizobacteria, Silicon, Solubilizing, IAA Production, Arachis hypogaea LAbstract
The aim of this investigation was to isolate groundnut root-associated rhizobacteria and to study their efficiency to solubilize silicate, synthesize indole acetic acid (IAA), encourage plant growth, and promote silicon (Si) uptake and deposit in plants. Based on silica-solubilizing ability and IAA production, a bacterial strain was chosen in the present study for further studies. The bacterial isolate synthesized more quantities of IAA at pH 8. When the soil fertilized with silica was inoculated with this bacterial strain, there was an improvement in overall growth characteristics of groundnut. It was also found that more Si was deposited in plants fertilized with both insoluble silica and bacterial strain. Bacteria isolated in the present study can synthesize more IAA under alkaline conditions, solubilize silicate, and encourage the growth of groundnut.
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References
Amies C A (1967). A modified formula for the preparation of Stuart’s Transport Medium - PubMed. Can J Public Heal., 296–300. Available at: https://pubmed.ncbi.nlm.nih.gov/4859908/ [Accessed July 14, 2022].
Calvaruso, C., Turpault, M. P., and Frey-Klett, P. (2006). Root-Associated Bacteria Contribute to Mineral Weathering and to Mineral Nutrition in Trees: a Budgeting Analysis. Appl. Environ. Microbiol. 72, 1258. doi: 10.1128/AEM.72.2.1258-1266.2006.
Etesami, H., and Jeong, B. R. (2022). Biodissolution of silica by rhizospheric silicate-solubilizing bacteria. Silicon Nano-silicon Environ. Stress Manag. Crop Qual. Improv., 265–276. doi: 10.1016/B978-0-323-91225-9.00020-0.
Jian, F. M., Tamai, K., Yamaji, N., Mitani, N., Konishi, S., Katsuhara, M., et al. (2006). A silicon transporter in rice. Nature 440, 688–691. doi: 10.1038/NATURE04590.
Kang, S.-M., Waqas, M., Shahzad, R., You, Y.-H., Asaf, S., Khan, M. A., et al. (2017). Soil Science and Plant Nutrition Isolation and characterization of a novel silicate-solubilizing bacterial strain Burkholderia eburnea CS4-2 that promotes growth of japonica rice (Oryza sativa L. cv. Dongjin) Isolation and characterization of a novel silicate-solubilizing bacterial strain Burkholderia eburnea CS4-2 that promotes growth of japonica rice (Oryza sativa L. cv. Dongjin). SOIL Sci. PLANT Nutr. 63, 233–241. doi: 10.1080/00380768.2017.1314829.
Khan, A. L., Waqas, M., Kang, S. M., Al-Harrasi, A., Hussain, J., Al-Rawahi, A., et al. (2014). Bacterial endophyte Sphingomonas sp. LK11 produces gibberellins and IAA and promotes tomato plant growth. J. Microbiol. 2014 528 52, 689–695. doi: 10.1007/S12275-014-4002-7.
Maheswari, R. U., Boominathan, A., and Dodagoudar, G. R. (2010). Use of surface waves in statistical correlations of shear wave velocity and penetration resistance of Chennai soils. Geotech. Geol. Eng. 28, 119–137. doi: 10.1007/S10706-009-9285-9.
Ribeiro, I. D. A., Volpiano, C. G., Vargas, L. K., Granada, C. E., Lisboa, B. B., and Passaglia, L. M. P. (2020). Use of Mineral Weathering Bacteria to Enhance Nutrient Availability in Crops: A Review. Front. Plant Sci. 11, 2047. doi: 10.3389/FPLS.2020.590774/BIBTEX.
Sahebi, M., Hanafi, M. M., Siti Nor Akmar, A., Rafii, M. Y., Azizi, P., and Idris, A. S. (2015). Serine-rich protein is a novel positive regulator for silicon accumulation in mangrove. Gene 556, 170–181. doi: 10.1016/J.GENE.2014.11.055.
Tamura, K., Dudley, J., Nei, M., and Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol. Biol. Evol. 24, 1596–1599. doi: 10.1093/MOLBEV/MSM092.
Taurian, T., Anzuay, M. S., Angelini, J. G., Tonelli, M. L., Ludueña, L., Pena, D., et al. (2010). Phosphate-solubilizing peanut associated bacteria: Screening for plant growth-promoting activities. Plant Soil 329, 421–431. doi: 10.1007/s11104-009-0168-x.
Vasanthi, N., and Rom, J. S. Evaluation of media for isolation and screening of silicate solubilising bacteria. Available at: http://www.journalcra.com [Accessed July 22, 2022].
Wang, X., Zhao, C., Müller, C., Wang, C., Ciais, P., Janssens, I., et al. (2020). Emergent constraint on crop yield response to warmer temperature from field experiments. Nat. Sustain. 2020 311 3, 908–916. doi: 10.1038/s41893-020-0569-7.
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