Extraction and Quantitative Determination of Cannabidiol (CBD) Fraction from Cannabis Sativa Species
DOI:
https://doi.org/10.55927/ijcs.v1i2.8259Keywords:
CBD, Cannabis SativaAbstract
Cannabidiol (CBD), non-psychoactive cannabinoid, was extracted from Cannabis sativa leaves by using solvent extraction method followed by reflux condenser procedure. Physical and chemical properties of Cannabidiol (CBD) were determined. Various parameters and contents of extracted oil were analyzed. Two different samples were prepared by applying different conditions. Sample “A” was prepared by applying reflux condenser and sample “B” was prepared by soaking eaves in ethanol (solvent) for one week. Various parameters and contents of extracted oil were analyzed. TLC, UV, GC-MS, IR and HPLC techniques were applied for characterization of both samples and oil. Some tests were also performed for the detection of proteins, carbohydrates, Steroids, tannins, gums and mucilage. Acid value, saponification number and specific gravity were also calculation by applying titration method. The results of both samples were showing major differences. The CBD content was also different in both samples. Due to non-psychoactive nature of CBD, it is known as safe side of marijuana and has many therapeutic benefits
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Abuhasira, R., Shbiro, L., & Landschaft, Y. (2018). Medical use of cannabis and cannabinoids containing products–Regulations in Europe and North America. European journal of internal medicine, 49, 2-6.
Atalay, S., Jarocka-Karpowicz, I., & Skrzydlewska, E. (2020). Antioxidative and anti- inflammatory properties of cannabidiol. Antioxidants, 9(1), 21.
Audu, B. S., Ofojekwu, P. C., Ujah, A., & Ajima, M. N. O. (2014). Phytochemical, proximate composition, amino acid profile and characterization of Marijuana (Cannabis sativa L.). The Journal of Phytopharmacology, 3(1), 35-43.
Borges, R. S., Batista Jr, J., Viana, R. B., Baetas, A. C., Orestes, E., Andrade, M. A., ... & Da Silva, A. B. (2013). Understanding the molecular aspects of tetrahydrocannabinol and cannabidiol as antioxidants. Molecules, 18(10), 12663-12674.
Brighenti, V., Marchetti, L., Anceschi, L., Protti, M., Verri, P., Pollastro, F., ... & Pellati, F. (2021). Separation and non-separation methods for the analysis of cannabinoids in Cannabis sativa L. Journal of Pharmaceutical and Biomedical Analysis, 206, 114346.
Corroon, J., & Phillips, J. A. (2018). A cross-sectional study of cannabidiol users. Cannabis and cannabinoid research, 3(1), 152-161.
ElSohly, M. A., Radwan, M. M., Gul, W., Chandra, S., & Galal, A. (2017). Phytochemistry of Cannabis sativa L. Phytocannabinoids, 1-36.
Ghovanloo, M. R., Shuart, N. G., Mezeyova, J., Dean, R. A., Ruben, P. C., & Goodchild, S. J. (2018). Inhibitory effects of cannabidiol on voltage-dependent sodium currents. Journal of Biological Chemistry, 293(43), 16546-16558.
Haldhar, R., Prasad, D., Mandal, N., Benhiba, F., Bahadur, I., & Dagdag, O. (2021). Anticorrosive properties of a green and sustainable inhibitor from leaves extract of Cannabis sativa plant: Experimental and theoretical approach. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 614, 126211.
Keating, G. M. (2017). Delta-9-tetrahydrocannabinol/cannabidiol oromucosal spray ( Sativex®): a review in multiple sclerosis-related spasticity. Drugs, 77, 563-574.
Li, H., Liu, Y., Tian, D., Tian, L., Ju, X., Qi, L., ... & Liang, C. (2020). Overview of cannabidiol (CBD) and its analogues: Structures, biological activities, and neuroprotective mechanisms in epilepsy and Alzheimer’s disease. European journal of medicinal chemistry, 192, 112163.
Nelson, K. M., Bisson, J., Singh, G., Graham, J. G., Chen, S. N., Friesen, J. B., ... & Pauli, G. F. (2020). The essential medicinal chemistry of cannabidiol (CBD). Journal of medicinal chemistry, 63(21), 12137-12155.
O’Dell, C. P., Tuell, D. S., Shah, D. S., & Stone, W. L. (2022). The systems medicine of cannabinoids in pediatrics: the case for more pediatric studies. Frontiers in Bioscience-Landmark, 27(1), 14.
Ryu, B. R., Islam, M. J., Azad, M. O. K., Go, E. J., Rahman, M. H., Rana, M. S., ... & Lim, J. D. (2021). Conversion Characteristics of some major cannabinoids from hemp (Cannabis sativa L.) raw materials by new rapid simultaneous analysis method. Molecules, 26(14), 4113.
Schilling, S., Melzer, R., & McCabe, P. F. (2020). Cannabis sativa. Current Biology, 30(1), R8-R9.
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