Optimisation and Characterisation of Curcumin Nanoparticles with Chitosan-TPP Combination Using Ionic Gelation Method as a Cut Wound Healer

Ferdinta D setyawan; Ilham  Kuncahyo; Wiwin  Herdwiani

doi:10.55927/eajmr.v3i9.10865

Authors

Ferdinta D setyawan Department of Pharmacy, Faculty of Pharmacy, Setiabudi University, Surakarta
Ilham Kuncahyo Department of Pharmacy, Faculty of Pharmacy, Setiabudi University, Surakarta
Wiwin Herdwiani Department of Pharmacy, Faculty of Pharmacy, Setiabudi University, Surakarta

DOI:

https://doi.org/10.55927/eajmr.v3i9.10865

Keywords:

Chitosan-TPP, Ionic Gelation, Nano Curcumin, Optimisation, Simplex Lattice Design

Abstract

Curcumin has low oral bioavailability, low solubility, and is easily degraded, making its clinical application difficult. This study aims to determine the effect of optimum concentration and formula of chitosan and TPP in the preparation of curcumin nanoparticles on particle size, zeta potential, adsorption efficiency, and incision wound healing. The optimisation method used simplex lattice design method. There were 8 draft formulas consisting of a combination of chitosan and TPP. Each formula was tested for zeta potential value, adsorption efficiency, particle morphology, and wound healing ability. The optimum formula was then determined and analysed using the simplex lattice design method. The combination of chitosan and TPP with simplex lattice design gives an influence on curcumin nanoparticles which reduces particle size, gives a negative zeta potential value, increases the sorption efficiency of the active substance (curcumin) and accelerates the healing effect of cut wounds in mice. The proportion of chitosan and TPP that can produce the optimum formula using simplex lattice design on the critical parameters of particle size, zeta potential value, sorption efficiency and wound healing ability is chitosan.

References

Abdassah, Marline. 2017. “Nanoparticles by Ionic Gelation Farmaka; 15 (1): 45-52.” 15: 45–52.

C.Gong, Q. Wu, Y. Wang, D. Zhang, F. Luo, X. Zhao, Y. Wei, Z. Qian. A biodegradable hydrogel system containing curcumin encapsulated in micelles for cutaneous wound healing. Biomaterials. 34 (2013) 6377-6387.

Cheng KK, Yeung CF, Ho SW, Chow, SF, Chow AHL, Baum L. Highly Stabilized Curcumin Nanoparticles Tested in an In Vitro Blood – Brain Barrier Model and in Alzheimer’s Disease Tg2576 Mice, AAPS Journal, 2012, 15(2); 324-335.

Chopra, Hitesh et al. 2021. “Curcumin Nanoparticles as Promising Therapeutic Agents for Drug Targets.” Molecules 26(16): 4998.

D. Akbik, M. Ghadiri, W. Chrzanowski, R. Rohanizadeh. Curcumin as a wound healing agent. Life Sciences. 116 (2014) 1–7.

E. Abd, S. Namjoshi, Y. H. Mohammed, M. S. Roberts and J. E. Grice, Synergistic skin penetration enhancer and nanoemulsion formulations promote the human epidermal permeation of caffeine and naproxen, J. Pharm. Sci., 2016, 105, 212–220.

F. Shakeel, S. Baboota, A. Ahuja, J. Ali, S. Shafiq. Celecoxib Nanoemulsion for Transdermal Drug Delivery: Characterization and In Vitro Evaluation, J. Disp. Sci. Technol. 6 (2009) 834-842.

G.S. Sidhu, A.K. Singh, D. Thaloor, K.K. Banaudha, G.K. Patnaik, R.C. Srimal, R.K. Maheshwari. Enhancement of wound healing by curcumin in animals, Wound Repair and Regeneration. 6 (1998) 167-178.

Guterres SS, Alves MP, Pohlmann AR. Polymeric Nanoparticles; Nanospheres and Nanocapsules for Cutaneosus Applications, Drug Target Insights, 2007, 2; 147–157.

H. P. Thakkar, A. Khunt, R. D. Dhande and A. A. Patel, Formulation and evaluation of Itraconazole nanoemulsion for enhanced oral bioavailability, J. Microencapsulation, 2015, 32, 559–569.

Juliantoni, Yohanes, Wahida Hajrin, and Windah Anugrah Subaidah. 2020. “Nanoparticle Formula Optimization of Juwet Seeds Extract (Syzygium Cumini) Using Simplex Lattice Design Method.” Jurnal Biologi Tropis 20(3): 416–22.

Kim TH, Jiang HH, Youn YS, Park CW, Tak KK, Lee S, Kim H, Jon S, Chen X and Lee KC. Preparation and characterization of water-soluble-albumin-bound curcumin nanoparticle with improved antitumor activity. Int. J. Pharm, 2012, 403; 285-291.

Krausz, A.E. Curcuminencapsulated nanoparticles as innovative antimicrobial and wound healing agent. Nanomedicine. 2015: 11(1): 195–206.

L. Thomas, F. Zakir, M. A. Mirza, M. K. Anwer, F. J. Ahmad and Z. Iqbal, Development of Curcumin loaded chitosan polymer based nanoemulsion gel: in vitro, ex vivo evaluation and in vivo wound healing studies, Int. J. Biol. Macromol., 2017, 101, 569–579.

Łuczak, Justyna et al. 2016. “Ionic Liquids for Nano- and Microstructures Preparation. Part 2: Application in Synthesis.” Advances in Colloid and Interface Science 227: 1–52. /retrieve/pii/S0001868615001517.

M. Laxmi, A. Bhardwaj, S. Mehta and A. Mehta, Development and characterization of nanoemulsion as carrier for the enhancement of bioavailability of artemether, Artif. Cells, Nanomed., Biotechnol., 2015, 43(5), 334–344.

Maesaroh, Umi, and Nanung Danar Dono. 2019. “Maesaroh, 2019.” 2(2): 91–95.

Martien, Ronny et al. 2012. “Technology Developments Nanoparticles as Drug.” Pharmaceutics Magazine 8(1): 133–44. https://article/view/24067/15747.

Mishra VK, Mohammad G, Mishra SK. Downregulation of Telomerase Activity May Enhanced by Nanoparticle Mediated Curcumin Delivery. Digest J. of Nano. Biostruct., 2008, 3; 163-169.

N. Ahmad, M. A. Alam, F. J. Ahmad, M. Sarafroz, K. Ansari, S. Sharma and M. Amir, Ultrasonication techniques used for the preparation of novel eugenol-nanoemulsion in the treatment of wounds healings and anti-inammatory, J. Drug Delivery Sci. Technol., 2018, 46, 461–473.

Ranjan AP, Mukerjee A, Helson L, Vishwanatha K. Scale Up, Optimization and Stability Analysis of Curcumin C3 Complex-Loaded Nanoparticles for Cancer Therapy, J. of Nanobiotech., 2012, 10(38); 1-18.

S. Shafiq, F. Shakeel, S. Talegaonkar, F.J. Ahmad, R.K. Khar, M. Ali. Development and bioavailability assessment of ramipril nanoemulsion formulation, Eur.J.Pharm. Biopharm. 66 (2007) 227-243.

Savitry, Putri Eka, and Nasrul Wathoni. 2018. “Characterization of sorption efficiency of diclofenac sodium nanoparticles in topical preparations.” Farmaka 16(2): 493–507.

Suryani, Martien R, Ismail H. Preparation of Curcumin Nanoparticles and Cellular Uptake Study on HeLa Cells, Proceeding International Seminar Latest Trends In Food,Biological and Ecological Science, Dubai October 11-12th, 2015;13-17.

Suryani., Wahyuni., Dian, Ariastika., Rahmanpiu. (2016). Formulation of Curcumin Nanoparticles by Ionic Gelation Technique Using Chitosan, Tripolyphosphate and Sodium Alginate and In Vitro Stability Testing. 2(1). 19-20.

T. Tadros, P. Izquierdo, J. Esquena and C. Solans, Formation and stability of nano-emulsions, Adv. Colloid Interface Sci., 2004, 108–109, 303–318.

Vimala, K. Fabrication of Curcumin Encapsulated Chitosan-PVA Silver Nanocomposite Films for Improved Antimicrobial Activity. JBNB (2011) 2: 55-64. 12.

Wang, Ziying et al. 2021. “Recent Advances in Natural Functional Biopolymers and Their Applications of Electronic Skins and Flexible Strain Sensors.” Polymers 13(5): 813. https://www.mdpi.com/2073-4360/13/5/813.

Wulandary, T. (2010). Synthesis of Temulawak Extract Nanoparticles (Curcuma xanthorrhiza Roxb.) Chitosan-TPP Polymer Based with Emulsion Method. 2.

You JO, Liu YC, Peng CA. Efficient Gene Transfection Using Chitosan–Alginate Core-Shell Nanoparticles. Int. J. of Nanomed., 2006, 1(2); 173–180.

Yusuf, Helmy, Rizka Arifa Rahmawati, M Agus Syamsur Rijal, and Dewi Isadiartuti. 2021. “Curcumin Micelles Entrapped in Eudragit S-100 Matrix: A Synergistic Strategy for Enhanced Oral Delivery.” Future Science OA 7(4). /doi/10.2144/fsoa-2020-0131.

Zorofchian Moghadamtousi, Soheil et al. 2014. “A Review on Antibacterial, Antiviral, and Antifungal Activity of Curcumin.” BioMed Research International 2014: 1–12.