Sampling of Sars-Cov-2 Covid-19 an Air Using the Impingement Method with Real-Time PCR as Detection Examination
DOI:
https://doi.org/10.55927/eajmr.v3i3.8651Keywords:
Covid-19, Air Sampling, Impingement MethodAbstract
Examination of airborne microbial for SARS-CoV-2, Coronavirus Disease 2019 (Covid-19) has not been widely developed. Whether this virus is airborne is still an unanswered question. This preliminary study was to evaluate the presence of the Covid-19 in the air. The study was conducted on 4 rooms, which are laboratory rooms used for Covid-19 activities. The impingement which is an active method used for air sampling, while the detection was carried out through a molecular approach using RT real-time PCR. The overall Covid-19 virus was not found in the room being examined. Four rooms gave CT values above the positive control, that is administration room (CT 30.44), BSL room (CT 30.23), BSC (CT 30.69 ) and waiting room (CT 35.61). No found SARS-CoV-2 Covid-19 in air using the impingement method with real-time PCR as detection examination in the room being examination. Preliminary study shown SARS-CoV-2 Covid-19 can not transmitted through the air. Research needs to be done with more sampling as continuation of this study.
References
Advice on the use of masks in the context of COVID-19. Interim guidance. Jenewa: World Health Organization; 2020. Available from https://www.who.int/publications/i/item/advice-on-the-use-of-masks-in-the-community-during-home-careand- in-healthcare-settings-in-the-context- of- the-novel-coronavirus-(2019-ncov)-outbreak)
Ali RR , Mostafa L , Ghasem A , Ali P. 2020. Sampling and detection of corona viruses in air: A mini review. Science of the Total Environment, 740: 140207
Anies. 2020. COVID-19: Ins and Outs of Corona Virus (Overview from the Aspect of Public Health). Yogyakarta: Arruzz Media.
Bullard J, Dust K, Funk D, Strong JE, Alexander D, Garnett L, et al. 2020. Predicting infectious SARS-CoV- 2 from diagnostic samples. Clin Infect Dis, 2020:ciaa638.
Cheng VC-C, Wong S-C, Chan VW-M, So SY-C, Chen JH-K, Yip CC-Y, et al. 2020. Air and environmental sampling for SARS- CoV-2 around hospitalized patients with coronavirus disease 2019 (COVID- 19). Infect Control Hosp Epidemiol. 1-32.
Chia PY, Coleman KK, Tan YK, Ong SWX Gum, M Lau SK, Sutjipto S, Lee PH, Young BE, Milton DK, 2020. Detection of Air and Surface Contamination by Severe Acute RespiratorySyndrome Coronavirus 2 (SARS-CoV-2) in Hospital Rooms of Infected Patients. Na Commun.11: 2800 classification-of-omicron-(b.1.1.529)-sars-cov-2- variant-of-concern
Cox J, Mbareche H, Lindsle YWG, Duchaine C. 2020. Field sampling of indoor bioaerosols. Aerosol Sci Tech, 54, 572–584
Daud A, Syam A, Arsin A SS. 2020. The handling of Coronavirus (Covid-19) from a perspective public health. Yogyakarta: Gosyen Publishing
Durante ME, Andini R, Bertolino L, Mele F, Bernardo M, Grimaldi M, et al. 2020. Low rate of
Faridi S, Niazi S, Sadeghi K, Naddafi K, Yavarian J, Shamsipour M, et al. 2020. A field indoor air measurement of SARS-CoV2 in the patient rooms of the largest hospital in Iran. Sci Total Environ, 725:138401.
Ghinai I, McPherson TD, Hunter JC, Kirking HL, Christiansen D, Joshi K, et al. 2020. First known person- to-person transmission of severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) in the USA. Lancet, 395:1137-44
Governur of Jakarta. 2020. Regulation of the Governor of the Special Capital Region of Jakarta No.3. Year 2021. Regarding the Implementation Regulations of Regional Regulation No. 2 of 2020 concerning Countermesures Corona Virus Disease 2019.
Guo ZD, Wang ZY, Zhang SF, Li X, Li L, Li C, et al. 2020. Aerosol and Surface Distribution of Severe Acute Respiratory Syndrome Coronavirus 2 in Hospital Wards, Wuhan, China. Emerg Infect Dis, 26(7).
Infection Prevention and Control of Epidemic-and Pandemic-prone Acute Respiratory Infections in Health Care. Jenewa: World Health Organization; 2014. Available from https://apps.who.int/iris/bitstream/handle/10665/112656/9789241507134_eng.pdf;j sessionid=41AA684FB64571CE8D8A 453 C4F2B2096?sequence=1)
Johns Hopkins University and Medicine. Covid-19 Dashboard by the Center for System Science and Engineering (CSSE) 2020. Baltimore:Johns Hopkins University and Medicine:2020.
Kominfo RI. Seven things to know about the Omricon variant that causes COVID-19 [poster]. Jakarta: Kominfo RI; 2021 [cited 2021 Dec 1]. Available from: https://covid19.go.id/edukasi/ masyarakat-umum/7-hal-yang-perlu-diketahuidari-varian-omicron-penyebab-covid-19
Leung M, Chan AH. 2006. Control and management of hospital indoor air quality. Med. Sci. Monit, 12(3):SR17–SR23
Lindsley WG, Green BJ, Blachere FM, Martin SB, Law B, Jensen P, Schafer M. 2020. Sampling and Characterization of Bioaerosols. NIOSH Manual of Analytical Methods. 5th ed. National Institute for Occupational Safety and Health, Cincinnati (OH).
Liu J, Liao X, Qian S, Yuan J, Wang F, Liu Y, et al. 2020. Community Transmission of Severe Acute Respiratory Syndrome Coronavirus 2, Shenzhen, China. Emerg Infect Dis, 26:1320-3.
Luo L, Liu D, Liao X, Wu X, Jing Q, Zheng J, et al. 2020. Modes of contact and risk of transmission in COVID-19 among close contacts (pracetak). MedRxiv. 2020 doi:10.1101/2020.03.24.20042606
Made AW, Mutria F, Ketut S, Wayan W, Gede J, Ni Wayan SB, Citranu C, Gusti NS, Wayan S. 2020. COVID-19: Legal and social perepectives of the We Write Foundation.. 2020; 0 [cited 2020 14 September]. Available form:https://scholar.google.com/scholar?hl=id&as_sdt=0%2C5&q= dampak+psbb+bagi+aspek+kehidupan+masyarakat&btnG=
Ministry of Health of the Republic of Indonesia. Current situation of Development of Coronavirus (Covid-19) : 15 Desember 2020. [cited 2020 26 Desember]. Available from: https://covid19.go.id/peta-sebaran
Ministry of Health of the Republic of Indonesia. Decree of the Minister of Health of the Republic of Indonesia number hk.01.07/ministry of helath/413/2020 concerning guidelines for prevention and control. Ministry of Health of the Republic of Indonesia. Jakarta : 2020
Nasution DAD, Erlina MI. 2020. The impact of the Cpvid-19 pandemic on Indonesia economy. J Benefita, 5(2):212.
Ong SWX, Tan YK, Chia PY, Lee TH, Ng OT, Wong MSY, et al. 2020. Air, Surface Environmental, and Personal Protective Equipment Contamination by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) From a Symptomatic Patient. JAMA, 323(16):1610- 1612 ordinary personal protection equipment in a medium- incidence setting. Clin Microbi Infect.2020:S1198743X20302706.
Pan M, Lednicky JA, Wu CY. 2019. Collection, particle sizing and detection of airborne viruses. J. Appl. Microbiol, 127(6):1596–1611. severe acute respiratory syndrome coronavirus 2 spread among health-care personnel using
Torjesen I. 2021. Covid-19: Omicron may be more transmissible than other variants and partly resistant to existing vaccines, scientists fear. BMJ, 375;2943.
Verreault D, Moineau S, Duchaine C. 2022 Methods for sampling of airborne viruses. Microbiol Mol. Biol Rev, (3), 413-444.
WHO. Classification of Omicron (B.1.1.529): SARS-CoV-2 Variant of Concern [Internet].
WHO; 2021 [cited 2021 Dec 7]. Available from: https://www.who.int/news/item/26-11-2021-
World Health Organization. Coronavirus disease 2019 (COVID-19) Situation Report – 42. World Health Organization. 2020. [cited 2020 26 Desember]:[62 p.]. Available from: https://www.who.int/publications/i/item/clinicalmanagement-of-covid-19
World Health Organization. Coronavirus Disease 2019 (COVID-19) Situation Report – 7 2020. 2020; 0 [cited 2020 14 September]. Available from: https://www.who.int/docs/defaultsource/coronaviruse/situationreports/202003 02-sitrep-42- covid-19.pdf?sfvrsn=224c1add_2
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